filtering differentially expressed genes with Bioconductor??

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    baumeist
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    Hi,

    I am relatively new to R and Bioconductor and am trying to filter the topTable that I generated of differentially expressed genes from my normlized eset file comprised of ~ 40 HG-133A Affy microarrays . I would like to see if particular probesets are represented in this list. Alternatively I would like to
    generate a topTable of differentially expressed genes using only specified probesets within my eset
    file.

    Can anyone tell me how I would begin to approach this? I have looked into using the genefilter() function but can’t figure out if it can take the right parameters (i.e. specific probe set id’s).

    Thanks in advance,
    -M

    This is the code I used to generate my topTable

    > fit <- lmFit(eset, design) > cont.matrix <- makeContrasts(NormalvsTumor=Tumor-Normal, levels=design) > fit2 <- contrasts.fit(fit, cont.matrix) > fit2 <- eBayes(fit2) > topTable(fit2, number=100, adjust=”BH”)

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